Culturing the moss Physcomitrella patens.
نویسندگان
چکیده
This article includes a series of methods for culturing the moss Physcomitrella patens at all stages of its life cycle. Gametophytes are axenically cultured on solid agar-based media and in shaken liquid cultures. For long-term storage of gametophytes, cultures are maintained on solid medium at 10°C in a very short day. Cryopreservation may also be used. Finally, sporophytes are generated by self-fertilization and sexual crossing.
منابع مشابه
Transformation of moss Physcomitrella patens gametophytes using a biolistic projectile delivery system.
RELATED INFORMATION For more information about P. patens as a model organism, see The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies (Cove et al. 2009a). The growth of protonemal tissue is described in Culturing the Moss Physcomitrella patens (Cove et al. 2009b), and a method for isolation of P. patens protoplasts is found in Isolation and Regeneratio...
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عنوان ژورنال:
- Cold Spring Harbor protocols
دوره 2009 2 شماره
صفحات -
تاریخ انتشار 2009